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Series GSE128965 Query DataSets for GSE128965
Status Public on Apr 12, 2019
Title Long noncoding RNA ELIT-1 acts as a Smad3 cofactor to facilitate TGF-β/Smad signaling and promote epithelial-mesenchymal transition
Organism Homo sapiens
Experiment type Expression profiling by array
Summary TGF-β is involved in various biological processes, including development, differentiation, growth regulation, and epithelial-mesenchymal transition (EMT). In TGF-β/Smad signaling, receptor-activated Smad complexes activate or repress their target gene promoters. Smad cofactors are a group of Smad-binding proteins that promote recruitment of Smad complexes to these promoters. Long noncoding RNAs (lncRNAs), that behave as Smad cofactors have thus far not been identified. Here, we characterize a novel lncRNA EMT-associated lncRNA induced by TGF-β-1(ELIT-1). ELIT-1 was induced by TGF-β-stimulation via the TGF-β/Smad pathway in TGF-β-responsive cell lines. ELIT-1-depletion abrogated TGF-β-mediated EMT progression and expression of TGF-β target genes including Snail, a transcription factor critical for EMT. A positive correlation between high expression of ELIT-1 and poor prognosis in lung adenocarcinoma and gastric cancer patients suggests that ELIT-1 may be useful as a prognostic and therapeutic target. RIP assays revealed that ELIT-1 bound to Smad3, but not Smad2. In conjunction with Smad3, ELIT-1 enhanced Smad-responsive promoter activities by recruiting Smad3 to the promoters of its target genes including Snail, other TGF-β-target genes, and ELIT-1 itself. Collectively, these data show that ELIT-1 is a novel trans-acting lncRNA that forms a positive feedback loop to enhance TGF-β/Smad3 signaling and promote EMT progression.
Overall design To identify the genes induced by HBV replication, HBV genome containing episomal vectors (Ae, Bj35, Bj56) or its empty vector (pEB) were transfected in liver carcinoma cell line Huh7. After puromycin screening, the transfectants were subjected to purify its total RNA and followed by microarray analysis.
Contributor(s) Ohhata T, Sakai S, Kitagawa M
Citation(s) 30952633
Submission date Mar 28, 2019
Last update date Apr 13, 2019
Contact name Tatsuya Ohhata
Organization name Hamamatsu University School of Medicine
Department Department of Molecular Biology
Street address Higashi-ku Handayama 1-20-1
City Hamamatsu
State/province Shizuoka
ZIP/Postal code 431-3192
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (5)
GSM3689236 pEB_1
GSM3689237 pEB_2
GSM3689238 Ae
BioProject PRJNA529580

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128965_RAW.tar 62.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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