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Series GSE128834 Query DataSets for GSE128834
Status Public on Nov 17, 2019
Title The hematopoietic master transcription factor PU.1 requires its interaction with the SWI/SNF remodeler to access chromatin de novo [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary PU.1 is a prototype master transcription factor (TF) of hematopoietic cell differentiation with diverse roles in different lineages. Analysis of its genome-wide binding pattern across PU.1 expressing cell types revealed manifold cell type-specific binding patterns. They are not consistent with the epigenetic and chromatin constraints to PU.1 binding observed in vitro, suggesting that PU.1 requires auxiliary factors to access DNA in vivo. Using a model of transient mRNA expression we show that PU.1 induction leads to the extensive remodeling of chromatin, redistribution of partner transcription factors, and rapid initiation of a myeloid gene expression program in heterologous cell types. By probing mutant PU.1 variants for defects in chromatin access and screening for PU.1 proximal proteins in vivo, we found that its N-terminal acidic domain was required for the recruitment of SWI/SNF remodeling complexes, de novo chromatin access and stable binding as well as the redistribution of partner TFs.
Overall design ChIP-seq data from cells transiently mRNA transfected with the transcription factor PU.1 and mutants thereof
Contributor(s) Minderjahn J, Rehli M
Citation(s) 31964861
Submission date Mar 25, 2019
Last update date Jan 27, 2020
Contact name Michael Rehli
Organization name University Hospital Regensburg
Department Internal Med III
Street address F.-J.-Strauss-Allee 11
City Regensburg
ZIP/Postal code 93042
Country Germany
Platforms (3)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL15433 Illumina HiSeq 1000 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (55)
GSM3686918 BC_PU.1-ChIP_donorA
GSM3686919 BC_PU.1-ChIP_donorC
GSM3686920 cMO_PU.1-ChIP_donorA
This SubSeries is part of SuperSeries:
GSE128837 The hematopoietic master transcription factor PU.1 requires its interaction with the SWI/SNF remodeler to access chromatin de novo
BioProject PRJNA528988
SRA SRP189395

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128834_RAW.tar 12.0 Gb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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