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| Status |
Public on Mar 22, 2020 |
| Title |
RNA-seq analysis of dolastatin 15 treatment in HCT116 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Dolastatin 15 is a known tubulin binding agent and shows remarkable differential cytotoxicity against a panel of colorectal HCT116 isogenic cells. The compound causes anti-vascularization effect in human endothelial cells (HUVEC) in a dose dependent manner. Antiangiogenic effect was further validated in a zebrafish genetic model with activated HIF
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| |
|
| Overall design |
Examination of Dolastatin 15 effect on HCT116 cells relative to solvent controls using two different concentrations of 20 nM and 100 nM at 16 and 24 hour time points
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| |
|
| Contributor(s) |
Luesch H, Ratnayake R |
| Citation(s) |
32237262 |
| Submission date |
Mar 23, 2019 |
| Last update date |
Jun 24, 2020 |
| Contact name |
Tongjun Gu |
| E-mail(s) |
tgu@ufl.edu
|
| Organization name |
The University of Florida
|
| Department |
ICBR
|
| Street address |
2033 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (12)
|
| GSM3684373 |
Dolastatin 15; 20 nM treatment at 16H sample 1 |
| GSM3684374 |
Dolastatin 15; 20 nM treatment at 16H sample 2 |
| GSM3684375 |
Dolastatin 15; 100 nM treatment at 16H sample 1 |
| GSM3684376 |
Dolastatin 15; 100 nM treatment at 16H sample 2 |
| GSM3684377 |
Dolastatin 15; solvent control for 16H sample 1 |
| GSM3684378 |
Dolastatin 15; solvent control for 16H sample 2 |
| GSM3684379 |
Dolastatin 15; 20 nM treatment at 24H sample 1 |
| GSM3684380 |
Dolastatin 15; 20 nM treatment at 24H sample 2 |
| GSM3684381 |
Dolastatin 15; 100 nM treatment at 24H sample 1 |
| GSM3684382 |
Dolastatin 15; 100 nM treatment at 24H sample 2 |
| GSM3684383 |
Dolastatin 15; solvent control for 24H sample 1 |
| GSM3684384 |
Dolastatin 15; solvent control for 24H sample 2 |
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| Relations |
| BioProject |
PRJNA528759 |
| SRA |
SRP189242 |
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