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Series GSE128671 Query DataSets for GSE128671
Status Public on Jan 17, 2020
Title Replicational dilution of H3K27me3 in mammalian cells and the role of poised promoters
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Differential effect of PRC2 loss on gene repression in intestinal stem cells, transit amplifying cells, and differentiated villus cells in intestine is explained by disparity in the rate of cell division and resulting difference in H3K27me3 loss.
 
Overall design We conducted RNA-seq, ChIP-seq, scRNA-seq experiments using purified intestinal epithleal cells from wild-type and mutant adult mice and human lymphoma cell line.
 
Contributor(s) Jadhav U, Manieri E, Nalapareddy K, Madha S, Chakrabarti S, Wucherpfennig K, Barefoot M, Shivdasani RA
Citation(s) 32027840
Submission date Mar 21, 2019
Last update date Feb 07, 2020
Contact name Ramesh Shivdasani
E-mail(s) ramesh_shivdasani@dfci.harvard.edu
Organization name Dana Farber Cancer Institute
Department Medical Oncology
Lab Shivdasani
Street address 450 Brookline Ave. Dana 720D
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (3)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (47)
GSM3682071 WT Adult Intestinal Villus H3K27me3 ChIP Rep1
GSM3682072 WT Adult Intestinal_Villus H3K27me3 ChIP Rep2
GSM3682073 Eed KO Adult Intestinal Villus H3K27me3 ChIP Rep1
Relations
BioProject PRJNA528482
SRA SRP189091

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128671_CountsTable_Mouse_Cells.txt.gz 340.0 Kb (ftp)(http) TXT
GSE128671_RAW.tar 4.8 Gb (http)(custom) TAR (of BW, MTX, TSV)
GSE128671_SUDHL10_RNAseq_Raw_Read_Counts.txt.gz 458.1 Kb (ftp)(http) TXT
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Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA
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