|
| Status |
Public on May 08, 2019 |
| Title |
Aberrant expression of select piRNA-pathway genes does not reactivate piRNA silencing in cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Analysis of PIWIL1 function in COLO205 [ATCC® CCL-222] colon adenocarcinoma cancer cell culture model.
|
| |
|
| Overall design |
Total Small RNAs and small RNAs co-immunoprecipitated with PIWIL1 from COLO205 cells were analyzed for piRNA signatures. Total RNA sequencing from PIWIL1 short hairpin knock-downs and CRISPR-generated knockouts were used for differential gene and transposon expression.
|
| |
|
| Contributor(s) |
Genzor P, Cordts SC, Bokil NV, Haase AD |
| Citation(s) |
31110013 |
| Submission date |
Mar 19, 2019 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Pavol Genzor |
| E-mail(s) |
pavol.genzor@gmail.com
|
| Organization name |
Henry M Jackson Foundation
|
| Department |
ACESO
|
| Street address |
6720A Rockledge Drive, Suite 100
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20817 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA528177 |
| SRA |
SRP188921 |
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