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Series GSE128469 Query DataSets for GSE128469
Status Public on Aug 27, 2019
Title Synchronized HES7 Oscillation In Human Embryonic Stem Cell-Derived Presomitic Mesoderm
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Little is known about human segmentation clock during somitogenesis. Human embryonic stem (ES) cell differentiation towards PSM and somite cells provide a window of opportunity to probe for the dynamics of oscillatory gene expression. Using high temporal RNA-seq, we captured a transcriptional signature highly reminiscent of segmentation clock during somite differentiation. Our study provides a novel in vitro system to model human segmentation clock that is otherwise inaccessible during development.
Overall design Human ES differentiation to PSM and somite cell fate, profiled by RNA-seq. Daily profiling, total 12 samples, triplicate for each day of sampling. 30 mins profiling, total 46 samples, replicates for each time points.
Contributor(s) Chu L
Citation(s) 31461642
Submission date Mar 18, 2019
Last update date Nov 04, 2019
Contact name Li-Fang Chu
Organization name Morgridge Institute for Research
Street address 330 N. Orchard St.
City Madison
ZIP/Postal code 53715
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (58)
GSM3676189 H1_Day0_replicate1
GSM3676190 H1_Day0_replicate2
GSM3676191 H1_Day0_replicate3
BioProject PRJNA527813
SRA SRP188722

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Supplementary file Size Download File type/resource 1.9 Mb (ftp)(http) TXT 1.9 Mb (ftp)(http) TXT
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