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| Status |
Public on Aug 27, 2019 |
| Title |
Synchronized HES7 Oscillation In Human Embryonic Stem Cell-Derived Presomitic Mesoderm |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Little is known about human segmentation clock during somitogenesis. Human embryonic stem (ES) cell differentiation towards PSM and somite cells provide a window of opportunity to probe for the dynamics of oscillatory gene expression. Using high temporal RNA-seq, we captured a transcriptional signature highly reminiscent of segmentation clock during somite differentiation. Our study provides a novel in vitro system to model human segmentation clock that is otherwise inaccessible during development.
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| Overall design |
Human ES differentiation to PSM and somite cell fate, profiled by RNA-seq. Daily profiling, total 12 samples, triplicate for each day of sampling. 30 mins profiling, total 46 samples, replicates for each time points.
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| Contributor(s) |
Chu L |
| Citation(s) |
31461642 |
| Submission date |
Mar 18, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
Li-Fang Chu |
| E-mail(s) |
lchu@morgridge.org
|
| Organization name |
Morgridge Institute for Research
|
| Street address |
330 N. Orchard St.
|
| City |
Madison |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (58)
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| Relations |
| BioProject |
PRJNA527813 |
| SRA |
SRP188722 |
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