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Series GSE128458 Query DataSets for GSE128458
Status Public on May 18, 2020
Title Enhancer reprogramming driven by high-order assemblies of transcription factors promotes phenotypic plasticity and breast cancer endocrine resistance [RNA-Seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cellular plasticity has emerged as an important mechanism of therapy resistance in cancers, yet the underlying molecular mechanisms remain unclear. Using an established breast cancer cellular model for endocrine resistance, we show that hormone resistance is associated with enhanced cellular plasticity, indicated by a general downregulation of luminal/epithelial differentiation markers and upregulation of basal/mesenchymal invasive markers. Our extensive omics studies including GRO-seq on enhancer landscapes demonstrate that the global enhancer gain/loss reprogramming driven by the differential interactions between ERĪ± and other oncogenic transcription factors (TFs), predominantly GATA3 and AP1, profoundly alter breast cancer transcriptional programs. Our functional studies in various biological systems support a coordinated role of GATA3 and AP1-mediated enhancer reprogramming in driving cellular plasticity to achieve endocrine resistance or cancer invasive progression. Thus, changes in TF-TF and TF-enhancer interactions can lead to genome-wide enhancer reprogramming, resulting in transcriptional dysregulations that promote plasticity and cancer therapy-resistance progression.
 
Overall design Total RNA extraction followed by high throughput sequencing (RNA-seq)
 
Contributor(s) Zhang Z, Bi M, Liu Z
Citation(s) 32424275
Submission date Mar 18, 2019
Last update date Aug 17, 2020
Contact name Zhijie Jason Liu
E-mail(s) liuz7@uthscsa.edu
Organization name Universality of Texas Health Science Center at San Antonio
Department Department of Molecular Medicine
Lab Liu lab
Street address 7703 Floyd Curl Drive
City San Antonio
State/province TEXAS
ZIP/Postal code 78229
Country USA
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (38)
GSM3675975 MCF7_rep1_RNAseq
GSM3675976 MCF7_rep2_RNAseq
GSM3675977 TamR_rep1_RNAseq
This SubSeries is part of SuperSeries:
GSE128460 Enhancer reprogramming driven by high-order assemblies of transcription factors promotes phenotypic plasticity and breast cancer endocrine resistance
Relations
BioProject PRJNA527798
SRA SRP188712

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128458_HBCX124_readCounts.txt.gz 204.0 Kb (ftp)(http) TXT
GSE128458_T47D_readCounts.txt.gz 308.0 Kb (ftp)(http) TXT
GSE128458_readCounts.txt.gz 890.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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