|
| Status |
Public on Dec 31, 2022 |
| Title |
Efficacy of small molecule inhibitors and genetic interference of cGAS-STING axis on development of the senescence-associated secretory phenotype (SASP) in human stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rapid advancements in next generation sequencing (NGS) have revolutionized system-based analysis of genome-wide expression, cellular pathways and stress responses. We performed this cGAS-STING-associated study to streamline the transcriptomic profiling (RNA-seq) of human stromal cells manifesting a typical senescence-associated secretory phenotype (SASP) in a DNA damage setting.
|
| |
|
| Overall design |
Overall transcript profiles of primary normal human prostate stromal cells (PSC27) were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
|
| |
|
| Contributor(s) |
Sun Y, Xu Q |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 18, 2019 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Yu Sun |
| E-mail(s) |
sunyu@sinh.ac.cn, submarinesuny@yahoo.com
|
| Phone |
86-21-54923302
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Shanghai Institute of Nutrition and Health
|
| Lab |
Cancer Resistance
|
| Street address |
320 Yueyang Rd, Life Sciences Building A #1508
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA527713 |
| SRA |
SRP188672 |
Less...
More...