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Series GSE128420 Query DataSets for GSE128420
Status Public on Dec 31, 2022
Title Efficacy of small molecule inhibitors and genetic interference of cGAS-STING axis on development of the senescence-associated secretory phenotype (SASP) in human stromal cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Rapid advancements in next generation sequencing (NGS) have revolutionized system-based analysis of genome-wide expression, cellular pathways and stress responses. We performed this cGAS-STING-associated study to streamline the transcriptomic profiling (RNA-seq) of human stromal cells manifesting a typical senescence-associated secretory phenotype (SASP) in a DNA damage setting.
Overall design Overall transcript profiles of primary normal human prostate stromal cells (PSC27) were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
Contributor(s) Sun Y, Xu Q
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Submission date Mar 18, 2019
Last update date Dec 31, 2022
Contact name Yu Sun
Phone 86-21-54923302
Organization name Chinese Academy of Sciences
Department Shanghai Institute of Nutrition and Health
Lab Cancer Resistance
Street address 320 Yueyang Rd, Life Sciences Building A #1508
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (18)
GSM3674149 PSC27-A-01
GSM3674150 PSC27-A-02
GSM3674151 PSC27-A-03
BioProject PRJNA527713
SRA SRP188672

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Supplementary file Size Download File type/resource
GSE128420_A.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
GSE128420_C.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
GSE128420_D.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
GSE128420_E.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
GSE128420_F.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
GSE128420_G.deseq2.annot.xls.gz 12.4 Mb (ftp)(http) XLS
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