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Series GSE128341 Query DataSets for GSE128341
Status Public on Aug 19, 2019
Title microRNA array analysis of differentiated HL60 cells with PfbA
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Previous studies indicate that host phagocytes are regulated by a complex combination of pattern recognition receptors-signaling and miRNA inductions. We performed microRNA array to determine whether S. pneumoniae PfbA suppresses phagocytosis via miRNA inductions.
 
Overall design We performed microRNA array analysis using neutrophil like-differentiated HL60 cells, S. pneumoniae strains and rPfbA. We compared rPfbA-treated and non-treated cells, wild type and ∆pfbA-infected cells, and ∆pfbA with rPfbA-infected and ∆pfbA solely infected cells. In each cell sample, 6 replicates were pooled and microRNA was purified from the pooled cells.
 
Citation(s) 31482074
Submission date Mar 15, 2019
Last update date Nov 18, 2019
Contact name Masaya Yamaguchi
Organization name Osaka University
Department Graduate School of Dentistry
Street address 1-8, Yamadaoka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (5)
GSM3671659 T4WT: dHL60_T4WT_infected_1h
GSM3671660 T4pfbA: dHL60_T4pfbAKO_infected_1h
GSM3671661 T4pfbA_r: dHL60_T4pfbAKOwithrPfbA_infected_1h
Relations
BioProject PRJNA527201

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128341_RAW.tar 3.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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