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Series GSE128313 Query DataSets for GSE128313
Status Public on May 08, 2019
Title Highly-motile versus unsorted MDA-MB-231 breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The challenge of predicting which patients with breast cancer will develop metastases leads to the overtreatment of patients with benign disease and to the inadequate treatment of the aggressive cancers. Here, we report the development and testing of a microfluidic assay that quantifies the abundance and proliferation of migratory cells in breast-cancer specimens, for the assessment of their metastatic propensity and for the rapid screening of potential antimetastatic therapeutics. On the basis of the key roles of cell motility and proliferation in cancer metastasis, the device accurately predicts the metastatic potential of breast-cancer cell lines and of patient-derived xenografts. Compared to unsorted cancer cells, highly motile cells isolated by the device exhibited similar tumourigenic potential but markedly increased metastatic propensity in vivo. RNA sequencing of the highly motile cells revealed an enrichment of motility-related and survival-related genes. The approach might be developed into a companion assay for the prediction of metastasis in patients and for the selection of effective therapeutic regimens.
Overall design RNA was isolated from samples of 1000 migratory or unsorted cells in triplicate
Contributor(s) Yankaskas CL, Thompson KN, Paul CD, Vitolo MI, Mistriotis P, Mahendra A, Bajpai VK, Shea DJ, Manto KM, Chai AC, Varadarajan N, Kontrogianni-Konstantopoulos A, Martin SS, Konstantopoulos K
Citation(s) 31061459
Submission date Mar 14, 2019
Last update date May 08, 2019
Contact name Konstantinos Konstantopoulos
Organization name Johns Hopkins University
Department Chemical and Biomolecular Engineering
Street address 3400 N Charles St
City Baltimore
State/province MD
ZIP/Postal code 21218
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (6)
GSM3671274 MDA-MB-231: Ctl_1
GSM3671275 MDA-MB-231: Ctl_2
GSM3671276 MDA-MB-231: Ctl_3
BioProject PRJNA527110
SRA SRP188447

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Supplementary file Size Download File type/resource
GSE128313_Gene_symbols_and_normalized_reads.xlsx 1.5 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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