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Series GSE128298 Query DataSets for GSE128298
Status Public on Aug 07, 2019
Title Translatome and transcriptome analysis of resting B and T cells from mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We isolated ex vivo resting state B and T lymphocytes from B6 mouse spleen using magnetic separation (Miltenyi). After 2 hours of rest in RPMI mediaum, these cells were either used to isolate total RNA for RNAseq library preparation or treated with cycloheximide and proceeded with RNase digestion and library preparation for ribosome profiling (Riboseq).
Overall design RNAseq and Riboseq for resting state total B and T cells.
Contributor(s) Arimbasseri GA, Dev G
Citation(s) 31318442
Submission date Mar 14, 2019
Last update date Aug 09, 2019
Contact name G Aneeshkumar Arimbasseri
Phone +91 9971986746
Organization name National Institute of Immunology
Department Molecular Genetics Laboratory
Lab Molecular Genetics Laboratory
Street address Aruna Asaf Ali Marg
City New Delhi
State/province Delhi
ZIP/Postal code 110067
Country India
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM3670842 B1: Riboseq (single-end sequencing)
GSM3670843 B3: Riboseq (single-end sequencing
GSM3670844 T1: Riboseq (single-end sequencing
BioProject PRJNA527039
SRA SRP188420

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128298_RAW.tar 1.3 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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