|
| Status |
Public on Aug 07, 2019 |
| Title |
Translatome and transcriptome analysis of resting B and T cells from mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We isolated ex vivo resting state B and T lymphocytes from B6 mouse spleen using magnetic separation (Miltenyi). After 2 hours of rest in RPMI mediaum, these cells were either used to isolate total RNA for RNAseq library preparation or treated with cycloheximide and proceeded with RNase digestion and library preparation for ribosome profiling (Riboseq).
|
| |
|
| Overall design |
RNAseq and Riboseq for resting state total B and T cells.
|
| |
|
| Contributor(s) |
Arimbasseri GA, Dev G |
| Citation(s) |
31318442 |
| |
| Submission date |
Mar 14, 2019 |
| Last update date |
Aug 09, 2019 |
| Contact name |
G Aneeshkumar Arimbasseri |
| E-mail(s) |
aneesh@nii.ac.in
|
| Phone |
+91 9971986746
|
| Organization name |
National Institute of Immunology
|
| Department |
Molecular Genetics Laboratory
|
| Lab |
Molecular Genetics Laboratory
|
| Street address |
Aruna Asaf Ali Marg
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA527039 |
| SRA |
SRP188420 |
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