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Series GSE128191 Query DataSets for GSE128191
Status Public on Jul 04, 2019
Title Gene expression profiling of human neural crest cells and smooth muscle cells treated with DMSO or Nutlin3a
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Activation of the p53 transcription factor triggers apoptosis only in certain cell types. Human neural crest cells (hNCCs), which can be generated from human embryonic stem cells, undergo apoptosis when treated with the p53 activating drug Nutlin3a. In contrast, when hNCCs are differentiated towards the smooth muscle cell lineage (early-hSMCS), they no longer undergo apoptosis in response to Nutlin3a treatment. Here, we sought to determine whether the transcriptional response to p53 activation differs between cells that are susceptible to p53-driven apoptosis and cells that are resistant to p53-driven apoptosis. To this end, we performed RNA-seq to assess the transcriptional response to Nutlin3a treatment in hNCCs and early-hSMCs.
Overall design RNA-seq was performed on hNCCs and early-hSMCs treated with DMSO (vehicle control) or Nutlin3a for 8 hours. n=3 per cell type and treatment.
Contributor(s) Bowen ME, Attardi LD
Citation(s) 31178404
Submission date Mar 12, 2019
Last update date Jul 04, 2019
Contact name Margot Elizabeth Bowen
Organization name Stanford University
Department Radiation Oncology
Lab Laura Attardi
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (12)
GSM3666631 hNCCs-DMSO-rep1
GSM3666632 hNCCs-Nutlin3a-rep1
GSM3666633 hSMCs-DMSO-rep1
BioProject PRJNA526696
SRA SRP188223

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Supplementary file Size Download File type/resource
GSE128191_Bowen_DeSeq2_NCCNutlin_vs_NCCDMSO.txt.gz 1.9 Mb (ftp)(http) TXT
GSE128191_Bowen_DeSeq2_SMCNutlin_vs_SMCDMSO.txt.gz 1.7 Mb (ftp)(http) TXT
GSE128191_Bowen_DeSeq2_SMC_vs_NCC.txt.gz 1.9 Mb (ftp)(http) TXT
GSE128191_Bowen_hNCCs_hSMCs_counts.txt.gz 964.8 Kb (ftp)(http) TXT
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