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Series GSE128163 Query DataSets for GSE128163
Status Public on Nov 21, 2019
Title The human fetal thymus generates effector invariant γδ T cells in a Lin28b-dependent manner
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In the mouse thymus, invariant γδ T cells are generated at well-defined times during development and acquire effector functions before exiting the thymus. However, whether such thymic programming can occur in human is not known. Here we analyzed human fetal and post-natal γδ thymocytes and investigated the role of hematopoietic-stem-and-precursor-cells (HSPC) in the generation of human γδ T cells. Unlike post-natal γδ thymocytes, fetal γδ thymocytes were functionally programmed (e.g. IFNγ, granzymes), expressed low levels of terminal-deoxynucleotidyl-transferase (TdT) and were highly enriched for invariant/public germline-encoded CDR3 sequences (TRGV8-TRJP1-CATWDTTGWFKIF, TRDV2-TRDD3-CACDTGGY and TRDV1-TRDD3-CALGELGD, previously shown to be expanded in cytomegalovirus infection) composed of short-homology-repeat-containing gene segments. Furthermore, these unique characteristics were due to an intrinsic property of fetal HSPC caused by high expression of the RNA-binding protein Lin28b. In conclusion, our data indicate that the human fetal thymus generates, in a HSPC/Lin28b-dependent manner, invariant γδ T cells with programmed effector functions.
Overall design analysis of human fetal and post-natal γδ thymocytes and the role of hematopoietic-stem-and-precursor-cells (HSPC) in the generation of human γδ T cells.
Contributor(s) Tieppo P, Papadopoulou M, Gatti D, McGovern N, Chan J, Gosselin F, Goetgeluk G, Weening K, Ma L, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D
Citation(s) 31816633, 36195611
Submission date Mar 12, 2019
Last update date Oct 27, 2022
Contact name David Vermijlen
Organization name Université Libre de Bruxelles
Street address Boulevard de Triomphe, accès 2 (CP205/3)
City Brussels
ZIP/Postal code 1050
Country Belgium
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM3665996 Sample 1_OP9DL1_FL_S1
GSM3665997 Sample 2_OP9DL1_FL_S2
GSM3665998 Sample 3_OP9DL1_FL_S3
BioProject PRJNA526634
SRA SRP188186

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128163_CPM_FT_PNT.csv.gz 1.6 Mb (ftp)(http) CSV
GSE128163_CPM_OP9DL1.csv.gz 1.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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