GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE127999 Query DataSets for GSE127999
Status Public on Sep 05, 2019
Title AMPK links acetyl-coA homeostasis to BET recruitment in acute myeloid leukemia: RNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Altered metabolism fuels two hallmark properties of cancer cells, unlimited proliferation and differentiation blockade. AMP-activated protein kinase (AMPK) is a master regulator of bioenergetics crucial for glucose metabolism in acute myeloid leukemia (AML) and its inhibition delays leukemogenesis, but whether the metabolic function of AMPK alters AML epigenome remains unknown. Here, we demonstrate that AMPK maintains the epigenome of MLL-rearranged AML by linking acetyl-CoA homeostasis to BET bromodomain protein recruitment to chromatin. AMPK deletion reduced acetyl-CoA and histone acetylation, displacing BET proteins from chromatin in leukemia-initiating cells (LICs). In both mouse and patient-derived xenograft AML models, treating with AMPK and BET inhibitors synergistically suppressed AML. Our results provide a therapeutic rationale to target AMPK and BET for AML therapy.
Overall design RNA-seq of sorted AMPK WT or KO leukemia-GMP cells, with three replicates of each.
Contributor(s) Jiang Y, Hoegenauer KA, Nakada D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 07, 2019
Last update date Sep 05, 2019
Contact name Daisuke Nakada
Organization name Baylor College of Medicine
Department Molecular and Human Genetics
Street address One Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM3660018 WT_LGMP_1
GSM3660019 WT_LGMP_2
GSM3660020 WT_LGMP_3
This SubSeries is part of SuperSeries:
GSE128001 AMPK links acetyl-coA homeostasis to BET recruitment in acute myeloid leukemia
BioProject PRJNA526027
SRA SRP187857

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127999_FPKM.tsv.gz 1.2 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap