|
| Status |
Public on Mar 06, 2022 |
| Title |
Effect of BCDIN3D knock-out on gene expression in Chronic Myelogenous Leukemia (CML) Hap1 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
WT and BCDIN3D knocked-out Hap1 cells were analysed by RNA- and microRNA-Seq to uncover the effect of BCDIN3D on specific microRNAs and their messenger RNA targets.
|
| |
|
| Overall design |
Hap1 WT and BCDIN3D knock-out cells were grown for 48h in IMDM+10%FBS+PSQ. RNA and protein was purified using the Norgen RNA/protein purification plus kit. Two biological replicates were tested. The same total RNA of RIN 10 was used for RNA-Seq and microRNA-Seq.
|
| |
|
| Contributor(s) |
Abell NS, Xhemalce B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 06, 2019 |
| Last update date |
Mar 06, 2022 |
| Contact name |
Blerta Xhemalce |
| Organization name |
University of Texas at Austin
|
| Street address |
2500 Speedway
|
| City |
Austin |
| State/province |
Texas |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA525893 |
| SRA |
SRP187785 |
Less...
More...