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Series GSE127712 Query DataSets for GSE127712
Status Public on May 31, 2019
Title Methylome profiling of GADD45 deficient mouse embryonic stem cells
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary In order to study the physiological impact of GADD45 proteins on DNA methylation, we compared the methylation levels of Gadd45a,b,g triple knockout (TKO) and wild-type (Control) mouse embryonic stem cells (mESC) via whole genome bisulfite sequencing (WGBS).
 
Overall design Gadd45a,b,g triple knockout (TKO) mouse embryonic stem cells (mESC) were generated using the CRISPR/Cas9 system. Control clones were generated using the same system but without specific gRNA. Genomic DNA was extracted from two Gadd45 TKO and two Control mESC clones and subjected to whole-genome bisulfite sequencing (WGBS).
 
Contributor(s) Schüle K, Leichsenring M, Andreani T, Vastolo V, Mallick M, Musheev M, Karaulanov E, Niehrs C
Citation(s) 31171699
Submission date Mar 01, 2019
Last update date Jun 08, 2019
Contact name Emil Karaulanov
Organization name Institute of Molecular Biology
Lab Bioinformatics Core Facility
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM3637560 mESC_Control_clone5
GSM3637561 mESC_Control_clone283
GSM3637562 mESC_TKO_clone227
This SubSeries is part of SuperSeries:
GSE127720 GADD45 promotes locus specific DNA demethylation and 2C cycling in embryonic stem cells
Relations
BioProject PRJNA525089
SRA SRP187205

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127712_RAW.tar 896.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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