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Series GSE127664 Query DataSets for GSE127664
Status Public on Mar 02, 2019
Title RNA-seq experiment performed on activated human CD4+ T cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Analysis perfomed on four paired samples with two treatments and paired untreated sample
 
Overall design We purified naïve CD45RA+CD4+ T cells from PBMCs collected from SLE patients. These cells were maintained for two days in culture medium supplementd with 20 IU of IL-2. Thereafter cells were harvetd and total RNA was purified using the kit from InVitroen. The sequencing was perfomed using Ion Torrent Kit. The RNA-seq was perfomed on Ion Torrent Sequncer.
 
Contributor(s) Chauhan AK
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 01, 2019
Last update date Mar 03, 2019
Contact name Anil Kumar Chauhan
E-mail(s) chauhanakc0@gmail.com
Phone 314-477-9592
Organization name Saint Louis University
Department Medicine
Street address 1402 South grant
City Saint Louis
State/province MO
ZIP/Postal code 63104
Country USA
 
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (12)
GSM3636328 Subject 1 anti-CD3 + ICs
GSM3636329 Subject 1 anti-CD3 + anti-CD28
GSM3636330 Subject 1 anti-CD3
Relations
BioProject PRJNA525005
SRA SRP187181

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Supplementary file Size Download File type/resource
GSE127664_Combined_Analysis_Submit.xlsx.gz 9.9 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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