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Series GSE127512 Query DataSets for GSE127512
Status Public on Nov 17, 2020
Title UTX/KDM6A suppresses AP-1 and a gliogenesis program during neural differentiation of human pluripotent stem cells[ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary UTX/KDM6A is known to promote open chromatin structure to facilitate gene activation. Although UTX is important for normal and cancer development, its molecular activities in developmental gene regulation remain unclear. Using genetic knockout of UTX in human embryonic stem cells, we show that UTX promotes human pluripotency and suppresses neural differentiation. In human neural stem cells (hNSCs), UTX modulates self-renewal, is required for neuronal differentiation, and suppresses glial/astrocytic differentiation by positively and negatively regulating its target genes. 70% of UTX-occupied regions are associated with epigenetic modifications known to be influenced by UTX, and UTX knockout leads to significant changes in expression of more than 50% of its target genes but epigenetic changes in less than 6% of its target genes. UTX likely affects gene expression via alternative molecular activities in hNSCs. Our findings reveal new functions of UTX in gene regulation and cell fate decisions in human stem cells.
Overall design Examination of transcription factors as UTX/KDM6A binding sites in hNSC by ChIP-Seq, along with ATAC-Seq, ChIP-Seq of H3K4me3, H3K27me3 and H3K27ac in WT and UTX KO Neuronal Maturation cells. Intergrated analysis with transcriptome.
Contributor(s) Mulvey B, Xu B, C. Peng J
Citation(s) 32977832
Submission date Feb 28, 2019
Last update date Nov 17, 2020
Contact name Beisi Xu
Organization name St Jude Children's Research Hosipital
Department Center for Applied Bioinformatics
Street address 262 Danny Thomas Pl
City Memphis
State/province Tennessee
ZIP/Postal code 38105
Country USA
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (27)
GSM3635734 H3K27Ac-AB2-hNSC-H7-UTXKO
GSM3635735 H3K27Ac-AB2-hNSC-H7-WT
GSM3635736 H3K27Ac-AB2-hNSC-H9-UTXKO
This SubSeries is part of SuperSeries:
GSE127515 UTX/KDM6A suppresses AP-1 and a gliogenesis program during neural differentiation of human pluripotent stem cells
BioProject PRJNA524891
SRA SRP187097

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127512_H3K27Ac-AB2-hNSC-H7-UTXKO.hg19.narrowPeak.gz 584.0 Kb (ftp)(http) NARROWPEAK
GSE127512_H3K27Ac-AB2-hNSC-H7-WT.hg19.narrowPeak.gz 812.5 Kb (ftp)(http) NARROWPEAK
GSE127512_H3K27Ac-AB2-hNSC-H9-UTXKO.hg19.narrowPeak.gz 1.1 Mb (ftp)(http) NARROWPEAK
GSE127512_H3K27Ac-AB2-hNSC-H9-WT.hg19.narrowPeak.gz 1.1 Mb (ftp)(http) NARROWPEAK
GSE127512_H3K27me3.hNSC-UTXKO.Rep.SharpBroad.bed.gz 104.9 Kb (ftp)(http) BED
GSE127512_H3K27me3.hNSC-WT.Rep.SharpBroad.bed.gz 68.8 Kb (ftp)(http) BED
GSE127512_H3K4me3-AB2-hNSC-H7-UTXKO.hg19.narrowPeak.gz 395.0 Kb (ftp)(http) NARROWPEAK
GSE127512_H3K4me3-AB2-hNSC-H7-WT.hg19.narrowPeak.gz 463.8 Kb (ftp)(http) NARROWPEAK
GSE127512_H3K4me3-AB2-hNSC-H9-UTXKO.hg19.narrowPeak.gz 466.5 Kb (ftp)(http) NARROWPEAK
GSE127512_H3K4me3-AB2-hNSC-H9-WT.hg19.narrowPeak.gz 469.5 Kb (ftp)(http) NARROWPEAK
GSE127512_UTX-hNSC.SICER.bed.gz 135.6 Kb (ftp)(http) BED
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