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Series GSE127332 Query DataSets for GSE127332
Status Public on Dec 22, 2020
Title Global analysis of kidney gene expression by RNA-Seq of aged CTRP1-deficient mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aging affects multi-organ systems. What local and systemic factors influence renal structure and function in aging are not well understood. We and others have shown that the secretory protein, C1q/TNF-related protein 1 (CTRP1), regulates systemic metabolism and cardiovascular function. Whether CTRP1 has a role in kidney function is unknown. We provide evidence here that CTRP1 modulates renal physiology in an age- and sex-dependent manner. In addition to adipose tissue, CTRP1 is also expressed in kidney glomeruli, with podocyte being a major cell source. In mice lacking CTRP1, we observed significant increase in kidney weight and glomeruli hypertrophy in aged (~1 year) male, but not female or younger mice. Although glomerular filtration rate, plasma renin and aldosterone levels, and renal response to water restriction are not different between genotypes, aged CTRP1-deficient male mice have elevated blood pressure. Echocardiogram and pulse wave velocity measurements indicate normal heart function and vascular stiffness in CTRP1 knockout animals. Increased blood pressure is not due to greater salt retention. Paradoxically, CTRP1-deficient mice have elevated urinary sodium and potassium excretion, resulting, in part, from reduced expression of genes involved in renal sodium and potassium reabsorption. Despite renal hypertrophy, gene expression related to inflammation, fibrosis, and oxidative stress are significantly lower in CTRP1-deficient mice. RNA sequencing and pathway analyses reveal additional alterations and enrichments of genes in metabolic processes in the kidney of CTRP1-null animals. These results highlight novel contributions of CTRP1 to aging-associated changes in renal structure and function
Overall design Kidney RNA sequenceing and bioinformatics analyses were performed on age-matched 49 week-old wildtype (WT) and CTRP1-/- mice, with 4 biological replicates for each genotype.
Contributor(s) Rodriguez S, Little HC, Shepard BD, Tan SY, Wolfe A, Cheema MU, Jandu S, Woodward OM, Talbot CC Jr, Berkowitz DE, Pluznick JL, Wong GW
Citation(s) 31908037
Submission date Feb 27, 2019
Last update date Dec 22, 2020
Contact name Guang William Wong
Organization name The Johns Hopkins University
Department School of Medicine
Lab Rangos Bldg 474
Street address 855 N Wolfe Street
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM3634063 M1WT_769
GSM3634064 M1WT_799
GSM3634065 M1WT_821
BioProject PRJNA524700
SRA SRP187060

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Supplementary file Size Download File type/resource
GSE127332_Wong_FPKM_Values.txt.gz 1.9 Mb (ftp)(http) TXT
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