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Series GSE127270 Query DataSets for GSE127270
Status Public on Dec 19, 2019
Title Transcriptional profiling of nutrient-balanced early trilineage differentiations derived from H9 hPSCs.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here, we show unexpected and crucial metabolic nodes through TCA flux and glutaminolysis that modulate germ layer specification. Previous reports have shown that sustained glycolytic flux though MYC regulation is required for ectoderm specification. Our data support the finding that the germ layers have distinct metabolic profiles, but through investigation into the role of OXPHOS in germ layer specification.
Overall design Bulk RNA-Sequencing of H9-derived hPSCs differentiated to early trilineage (mesoderm, endoderm, and ectoderm) using a nutrient-balanced media formulation. Samples represent n = 5-7 of each lineage (hPSC, ME, EN, and EC).
Contributor(s) Ahsan FM, Lu V, Teitell MA
Citation(s) 31189991
Submission date Feb 27, 2019
Last update date Dec 21, 2019
Contact name Fasih Mubtasim Ahsan
Organization name UCLA
Department Pathology and Laboratory Medicine
Lab Teitell Lab, MRL 4-567
Street address 675 Charles E. Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095-1732
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (24)
GSM3633410 Meso Rep 1
GSM3633411 Ecto Rep 1
GSM3633412 hPSC Rep 1
BioProject PRJNA524638
SRA SRP187040

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127270_GEO_rawcountmatrix.csv.gz 2.2 Mb (ftp)(http) CSV
GSE127270_GEO_samplemetadata.csv.gz 383 b (ftp)(http) CSV
GSE127270_RAW.tar 78.6 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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