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Series GSE127221 Query DataSets for GSE127221
Status Public on Mar 04, 2019
Title Expression profiling of PBMCs from VA-ECLS patients
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We profiled RNA expression by means of single cell RNA-Seq from a total of ~60,000 cells from 38 patients undergoing VA-ECMO due to acute heart failure (including acute exacerbation of chronic failure). Samples were taken at the time of initiation of ECMO (mean +/- 79 minutes from cannulation).
 
Overall design Survival analysis, 30 day survival, with cross sectional analysis of gene expression at start of VA-ECLS
Submitters plan to submit raw files to dbGaP to protect patient sequence privacy
 
Contributor(s) Kort EJ, Jovinge S
Citation Eric J. Kort, Matthew Weiland, Edgars Grins, Emily Eugster, Hsiao-yun Milliron, Catherine Kelty, Nabin Manandhar Shrestha, Tomasz Timek, Marzia Leacche, Stephen J Fitch, Theodore J Boeve, Greg Marco, Michael Dickinson, Penny Wilton, Stefan Jovinge. Single cell transcriptomics identifies immunologic priming related to extra corporeal life support survival. bioRxiv 568659; doi:10.1101/568659
Submission date Feb 26, 2019
Last update date Mar 19, 2019
Contact name Eric Kort
E-mail(s) eric.kort@vai.org
Phone 6162345442
Organization name Van Andel Research Institute
Lab Jovinge Lab
Street address 333 Bostwick NE
City Grand Rapids
State/province MI
ZIP/Postal code 49503
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (38)
GSM3631057 Patient 1
GSM3631058 Patient 2
GSM3631059 Patient 3
Relations
BioProject PRJNA524362

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127221_PBMC_merged_filtered_recoded.rds.gz 170.2 Mb (ftp)(http) RDS
GSE127221_PBMC_merged_filtered_recoded.txt.gz 116.4 Mb (ftp)(http) TXT
GSE127221_metadata.rds.gz 1.7 Kb (ftp)(http) RDS
GSE127221_metadata.txt.gz 791 b (ftp)(http) TXT
Raw data not provided for this record
Processed data are available on Series record

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