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Series GSE127200 Query DataSets for GSE127200
Status Public on Mar 15, 2019
Title Gene expression data from sorted primary human acute myeloid leukemia (AML) samples
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In vivo studies indicate that human acute myeloid leukaemia (AML) originates from subpopulations of neoplastic cells with stem cell properties, thereby laying the foundation for the cancer stem cell concept. For clinically apparent disease to occur, cancer-initiating cells must also escape immune control. Here we show that stemness and immune evasion are closely intertwined in AML. Patients with AML harbour leukaemic subpopulations that lack expression of ligands for the danger detector NKG2D, a critical mediator of immune surveillance, which stimulates anti-tumour immunity by cytotoxic lymphocytes such as natural killer (NK) cells. We compared NKG2DLneg and NKG2DLpos subfractions from n=5 patients (each measured in triplicates) and found that NKG2DLneg cells displayed amongst others stemness characteristics.Our findings identify immune privilege as a general feature of LSCs, and provide a conceptual framework for the integration of two central hypotheses of cancer development – the concepts of cancer stem cells and immune escape
Overall design Analysis of gene expression in FACS sorted AML fractions separated into NKG2DLpos and NKG2DLneg fractions from n=5 AML patients (3 CD34 expressing and 2 CD34 non-expressing AML patients) each performed in triplicates
Contributor(s) Konantz M, Lengerke C
Citation(s) 31316209
Submission date Feb 26, 2019
Last update date Jul 19, 2019
Contact name Claudia Lengerke
Organization name University Hospital Basel and University of Basel
Department Department of Biomedicine
Street address Hebelstr. 20
City Basel
ZIP/Postal code 4055
Country Switzerland
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (30)
GSM3630216 1a_NKG2DLpos
GSM3630217 1b_NKG2DLpos
GSM3630218 1c_NKG2DLpos
BioProject PRJNA524344

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Supplementary file Size Download File type/resource
GSE127200_RAW.tar 368.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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