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Series GSE126983 Query DataSets for GSE126983
Status Public on Feb 26, 2019
Title PTENα/β paradoxically promote carcinogenesis through WDR5-H3K4me3 axis [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary we report that USP9X and FBXW11 selectively regulate the stability of PTENα/β but not PTEN proteins by deubiqitination and ubiquitination respectively. USP9X promotes and FBXW11 suppresses tumorigenesis mediated by PTENα/β. In contrast to the current paradigm for PTEN as a tumor suppressor, PTENα/β promote tumorigenesis of cancer cells in a phosphatase-independent manner. Mechanistically, PTENα/β localized in the nucleus regulate expressions of tumor-promoting genes such as NOTCH3 in the similar way as the H3K4 presenter WDR5. Further, PTENα/β but not PTEN directly interact with WDR5 to promote trimethylation of H3K4 and maintain a tumor-promoting signature.
Overall design RNA sequencing in parental and PTENα/βKO-1 SMMC-7721 cell lines with or without USP9X depletion by two independent experiments; RNA sequencing in MiaPaCa2 cell lines with or without PTENα/β
Contributor(s) Shen S, Zhang C, Ge M, Dong S
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Submission date Feb 24, 2019
Last update date Feb 27, 2019
Contact name ge mengkai
Phone 15201867201
Organization name Shanghai Jiao Tong University School of Medicine (SJTU-SM)
Street address shanghai
City shanghai
State/province State...
ZIP/Postal code 13185541251gmk
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (10)
GSM3619376 MiaPaCa2_gNS
GSM3619377 MiaPaCa2_gPTENα/β
GSM3619378 SMMC-7721_gNS-gNS_1
This SubSeries is part of SuperSeries:
GSE126984 PTENα/β paradoxically promote carcinogenesis through WDR5-H3K4me3 axis
BioProject PRJNA524002
SRA SRP186728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126983_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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