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| Status |
Public on Apr 30, 2019 |
| Title |
Dragotcytosis: Elucidation of the Mechanism for Cryptococcus neoformans Macrophage-to-Macrophage Transfer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Abstract: Cryptococcus neoformans is a pathogenic yeast capable of a unique and intriguing form of cell-to-cell transfer between macrophage cells. The mechanism for cell-to-cell transfer is not understood. Here we imaged macrophages with CellTracker Green CMFDA-labeled cytosol to ascertain whether cytosol was shared between donor and acceptor macrophages. Analysis of several transfer events detected no transfer of cytosol from donor to acceptor macrophages. However, blocking Fc and complement receptors resulted in a major diminution of cell-to-cell transfer events. The timing cell-to-cell transfer (11.17 min) closely approximated the sum of phagocytosis (4.18 min) and exocytosis (6.71 min) times. We propose that macrophage cell-to-cell transfer represents a non-lytic exocytosis event followed by phagocytosis into a macrophage that is in close proximity and name this process Dragotcytosis (Dragot is a Greek surname meaning ‘Sentinel’) as it represents sharing of a microbe between two sentinel cells of the innate immune system.
Microarrays were used to assess whether incubation of Bone Marrow-derived macrophages (BMDMs) with receptor blocking antibodies had an effect on their transcriptional profiles.
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| Overall design |
BMDMs incubated with or without the Fc receptor blocking antibody were processed in triplicate for each condition.
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| Contributor(s) |
Dragotakes Q, Fu MS, Casadevall A |
| Citation missing |
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| Submission date |
Feb 23, 2019 |
| Last update date |
May 01, 2019 |
| Contact name |
Anne E. Jedlicka |
| Organization name |
Johns Hopkins University, School of Public Health
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| Department |
Molecular Microbiology and Immunology
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| Lab |
Genomic Analysis and Sequencing Core
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| Street address |
615 N. Wolfe street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL21810 |
Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Feature Number version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA523941 |
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