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Series GSE126778 Query DataSets for GSE126778
Status Public on Feb 16, 2022
Title A missense mutation in AIOLOS causes B cell deficiency by interfering with IKAROS
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary A missense mutation (G158R) in Ikzf3 gene resulted in defective generation of B cells in mice. Pre-B cells were profoundly decreased in homozygous mutant mice. Heterozygous mutant mice showed milder B cell developmental defects, and decreased B cells in the secondary lymphoid organs were observed. Ikzf3 encodes Aiolos, which makes heterodimer with Ikaros. Our findings indicate that the loss-of-function mutation in Aiolos hinders B cell development by interfering Ikaros' function via formation of heterodimers.
 
Overall design Sample 1-18: mRNA profiles of pre-pro B cells and pre-B cells in wild-type, heterozygous and homozygous Ikzf3 G158R mutant mice.
Sample 19-30: Genome-wide bindings of Ikaros and Aiolos in pre-B cells and thymocytes of wild-type and homozygous Ikzf3 G158R mutant mice.
 
Contributor(s) Yamashita M
Citation(s) 34155405
Submission date Feb 19, 2019
Last update date Feb 17, 2022
Contact name Kazuki Okuyama
E-mail(s) kazuki.okuyama@riken.jp
Organization name RIKEN Yokohama
Department IMS
Lab Transcriptional Regulation
Street address 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama
City Kanagawa
ZIP/Postal code 230-0045
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (30)
GSM3612735 RNA-seq_WT pre-pro B rep1
GSM3612736 RNA-seq_WT pre-pro B rep2
GSM3612737 RNA-seq_WT pre-pro B rep3
Relations
BioProject PRJNA523246
SRA SRP186311

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Supplementary file Size Download File type/resource
GSE126778_RAW.tar 2.3 Mb (http)(custom) TAR (of BROADPEAK, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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