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Series GSE126734 Query DataSets for GSE126734
Status Public on May 18, 2020
Title Mechanisms of stretch-mediated skin expansion at single cell resolution [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The ability of the skin to expand in response to stretching has, for decades, been exploited in reconstructive surgery. Several studies have investigated the response of stretching epidermal cells in vitro. However, it remains unclear how mechanical forces affect epidermal stem cell behaviour in vivo. Here, we develop a mouse model in which the temporal consequences of the stretching the skin epidermis can be studied. Using a multidisciplinary approach that combines clonal analysis and mathematical modelling, we show that mechanical force induces skin expansion by promoting the renewal of epidermal stem cells. This occurs through a structured response in which cell fates are coordinated locally by stem cells that switch between states primed for renewal or differentiation. Transcriptional and chromatin profiling identifies the gene regulatory networks modulated by mechanical force. Using a combination of pharmacological inhibition and several conditional gene loss-of-function mouse mutants, we dissect the signalling pathways that control force-mediated tissue expansion.
Overall design We used ATACseq to profile the chromatin landscape of basal cells isolated from the interfolliular epidermis during force-mediated tissue expansion. Interfollicolar basal epideramal cells form the back skin were FACS sorted for the positive expression of ITGA6 and negative expression of CD34. One CD1 mouse was subjected to force-mediated tissue expansion and an untreted animal was used as control. For each sample, 10000 basal cells were FACS sorted and used for ATAC preparation.
Contributor(s) Aragona M, Song Y, Blanpain C
Citation(s) 32728211
Submission date Feb 19, 2019
Last update date Aug 17, 2020
Contact name Cedric Blanpain
Organization name Université Libre De Bruxelles
Street address route de Lennik, 808
City Bruxelles
ZIP/Postal code B-1070
Country Belgium
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (2)
GSM3611924 Ctl_S18
GSM3611925 Exp-D2
This SubSeries is part of SuperSeries:
GSE126735 Stem cell dynamic and signalling controlling mechanical force mediated skin expansion
BioProject PRJNA523146
SRA SRP186251

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Supplementary file Size Download File type/resource
GSE126734_RAW.tar 253.2 Mb (http)(custom) TAR (of BED, CSV, TDF, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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