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Series GSE126723 Query DataSets for GSE126723
Status Public on Feb 20, 2019
Title Genome-wide identification and analysis of A-to-I RNA editing events in the malignantly transformed cell lines from BEP2D induced by α-particles radiation
Organism Homo sapiens
Experiment type Other
Summary Adenosine (A) to inosine (I) RNA editing is the most prevalent RNA editing mechanism in humans and play critical roles in tumorigenesis. However, the effects of radiation on RNA editing and the mechanisms of radiation-induced cancer were poorly understood. Here, we analyzed human bronchial epithelial BEP2D cells and radiation-induced malignantly transformed cells with next generation sequencing. By performing an integrated analysis of A-to-I RNA editing, we found that genome-encoded single-nucleotide polymorphisms (SNPs) might induce the downregulation of ADAR2 enzymes, and further caused the abnormal occurrence of RNA editing in malignantly transformed cells. These editing events were significantly enriched in differentially expressed genes between normal cells and cancer cells. In addition, oncogenes CTNNB1 and FN1 were highly edited and significantly overexpressed in cancer cells, thus may be responsible for the lung cancer progression. Our work provides a systematic analysis of RNA editing from lung tumor specimens with high-throughput RNA sequencing and DNA sequencing. Moreover, these results demonstrate further evidence for RNA editing as an important tumorigenesis mechanism.
Overall design we analyzed human bronchial epithelial BEP2D cells and radiation-induced malignantly transformed cells with next generation sequencing. Then we identified RNA editome by three identification method. We integrated these RNA editome to eight stages and record their position, reference, alter, strand, editing level.
Contributor(s) Liu Q, Zhao C, Chen H, Li H
Citation(s) 31158229
Submission date Feb 19, 2019
Last update date Jun 24, 2019
Contact name Chenghui Zhao
Phone 18800113206
Organization name Beijing Institute of Radiation Medicine
Street address Haidian Taiping Road 27
City Beijing
ZIP/Postal code 100850
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (9)
GSM3611827 BEP2D REP1
GSM3611828 BEP2D REP2
GSM3611829 BEP2D REP3
BioProject PRJNA523118
SRA SRP186229

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Supplementary file Size Download File type/resource
GSE126723_RAW.tar 870.0 Kb (http)(custom) TAR (of VCF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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