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Series GSE126636 Query DataSets for GSE126636
Status Public on Jan 07, 2020
Title The structural basis for cohesin/CTCF anchored loops [Hi-C]
Organism Homo sapiens
Experiment type Other
Summary Cohesin catalyses the folding of the genome into loops that are anchored by CTCF. The molecular mechanism of how cohesin and CTCF structure the 3D genome has remained unclear. Here we show that a segment within the CTCF N terminus interacts with the SA2-SCC1 subunits of cohesin. A 2.6 Å crystal structure of SA2-SCC1 in complex with CTCF reveals the molecular basis of the interaction. We demonstrate that this interaction is specifically required for CTCF-anchored loops and contributes to the positioning of cohesin at CTCF-binding sites. A similar motif is present in a number of established and novel cohesin ligands, including the cohesin release factor WAPL. Our data suggest that CTCF enables chromatin loop formation by protecting cohesin against loop release. These results provide fundamental insights into the molecular mechanism that enables dynamic regulation of chromatin folding by cohesin and CTCF.
Overall design Hi-C was performed in control and mutant CTCF (Y226A, F228A) HAP1 cells in duplicate.
Contributor(s) Haarhuis JH, Sedeño Cacciatore A, van Ruiten MS, Willems L, Teunissen H, de Wit E, Rowland BD
Citation(s) 31905366
Submission date Feb 15, 2019
Last update date Feb 21, 2020
Contact name Angela Sedeno Cacciatore
Organization name Netherlands Cancer Institute
Department Gene regulation
Lab Rowland lab
Street address Plesmanlaan 121
City Amsterdam
State/province Noord-Holland
ZIP/Postal code 1066 CX
Country Netherlands
Platforms (2)
GPL21697 NextSeq 550 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4052980 Hi-C_WT 1
GSM4052981 Hi-C_CTCF Y226A, F228A 1
GSM4052982 Hi-C_WT 2.1
This SubSeries is part of SuperSeries:
GSE126637 The structural basis for cohesin/CTCF anchored loops
BioProject PRJNA522782
SRA SRP186012

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Supplementary file Size Download File type/resource
GSE126636_HiC_CTCF.Y226A.F228A_merged_allValidPairs.txt.gz 6.2 Gb (ftp)(http) TXT
GSE126636_HiC_WT_merged_allValidPairs.txt.gz 7.0 Gb (ftp)(http) TXT
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