|
| Status |
Public on Jan 07, 2020 |
| Title |
The structural basis for cohesin/CTCF anchored loops [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cohesin catalyses the folding of the genome into loops that are anchored by CTCF. The molecular mechanism of how cohesin and CTCF structure the 3D genome has remained unclear. Here we show that a segment within the CTCF N terminus interacts with the SA2-SCC1 subunits of cohesin. A 2.6 Å crystal structure of SA2-SCC1 in complex with CTCF reveals the molecular basis of the interaction. We demonstrate that this interaction is specifically required for CTCF-anchored loops and contributes to the positioning of cohesin at CTCF-binding sites. A similar motif is present in a number of established and novel cohesin ligands, including the cohesin release factor WAPL. Our data suggest that CTCF enables chromatin loop formation by protecting cohesin against loop release. These results provide fundamental insights into the molecular mechanism that enables dynamic regulation of chromatin folding by cohesin and CTCF.
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| |
|
| Overall design |
RNA-Seq was performed in control and mutant CTCF (Y226A, F228A) HAP1 cells in triplicate.
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| |
|
| Contributor(s) |
Haarhuis JH, Sedeño Cacciatore A, Willems L, de Wit E, Rowland BD |
| Citation(s) |
31905366 |
| |
| Submission date |
Feb 15, 2019 |
| Last update date |
Feb 21, 2020 |
| Contact name |
Angela Sedeno Cacciatore |
| E-mail(s) |
a.sedeno.cacciatore@nki.nl, m.v.ruiten@nki.nl
|
| Organization name |
Netherlands Cancer Institute
|
| Department |
Gene regulation
|
| Lab |
Rowland lab
|
| Street address |
Plesmanlaan 121
|
| City |
Amsterdam |
| State/province |
Noord-Holland |
| ZIP/Postal code |
1066 CX |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE126637 |
The structural basis for cohesin/CTCF anchored loops |
|
| Relations |
| BioProject |
PRJNA522781 |
| SRA |
SRP186011 |
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