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Series GSE126577 Query DataSets for GSE126577
Status Public on Jan 05, 2020
Title Genome-wide maps of Qki-5 and PPARb in mouse oligodendrocytes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We found that there is a big overlap between the Qki5- and PPARb-binding events by ChIP-seq. Interestingly, the genomic distribution analyses of Qki-5- and PPARĪ²-binding events showed significant enrichment at the regions of the promoter/transcription start site (TSS) which were indicated by Pol II-binding events. The strong preference of the promoter/TSS co-occupancy suggested that Qki-5 might play an important role in PPARĪ²-mediated transcriptional regulation of its downstream genes.
Overall design Examination of genome-wide maps Qki-5 and PPARb in oligodendrocytes which are differentiated from neural stem cells or oligodendrocytes freshly isolated from mouse brains by ChIP-seq. Neural stem cells were differentiated in Neurobasal medium containing B-27, 2 mM GlutaMAX-I, 100 ng/mL insulin-like growth factor 1 (IGF-1), and 50 ng/mL 3,3',5-Triiodo-L-thyronine (T3). Oligodendrocytes were freshly isolated from mouse brains using O4 magnetic beads.
Contributor(s) He C, Zhou X, Lan F, Hu J
Citation(s) 32202512
Submission date Feb 14, 2019
Last update date Apr 05, 2020
Contact name Chenxi He
Organization name Fudan University
Street address 131 Dong'an Rd, Mingdao Bldg, Rm 512
City Shanghai
ZIP/Postal code 200032
Country China
Platforms (2)
GPL21273 HiSeq X Ten (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM3608054 iOL_HA-PPAR_Qki-5_ChIPseq
GSM3608055 iOL_HA-PPAR_PPARbeta_ChIPseq
GSM3608056 iOL_HA-PPAR_HA_ChIPseq
BioProject PRJNA522428
SRA SRP185910

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Supplementary file Size Download File type/resource
GSE126577_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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