GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE126487 Query DataSets for GSE126487
Status Public on Apr 11, 2019
Title Alterations in GRHL2-OVOL2-ZEB1 Axis and Aberrant Activation of Wnt Signaling Lead to Altered Gene Transcription in Posterior Polymorphous Corneal Dystrophy
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the molecular basis of posterior polymorphous corneal dystrophy (PPCD) by examining the transcriptome in an affected individuals with an OVOL2 promoter mutation c.307T>C (PPCD1).
Overall design Cornea endothelial tissue from a patient (P7), age 67, with PPCD were obtained when the patient underwent keratoplasty . The Descemet membrane and endothelium were placed onto a piece of filter paper and submerged in TRI Reagent (Thermo Fisher, Waltham, MA) for RNA isolation. Human cornea tissue from an age-matched donor (C7), age 63, was obtained from an commercial eye bank. Descemet’s membrane and corneal endothelium were stripped from the donor cornea using a published technique and placed into TRI Reagent for RNA isolation.
Contributor(s) Chung DD, Aldave AJ
Citation(s) 31233731
Submission date Feb 13, 2019
Last update date Jul 12, 2019
Contact name Anthony John Aldave
Phone 3108256232
Organization name University of California, Los Angeles
Department Ophthalmology
Lab Aldave Lab
Street address 200 Stein Plaza, DS 3-143
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (2)
GSM3602448 PPCD CDDL 91 (P7)
GSM3602449 Control 223 (C7)
BioProject PRJNA522067
SRA SRP185780

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126487_Processed_Data_P7_C7.xls.gz 1.5 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap