|
Status |
Public on Feb 11, 2019 |
Title |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 [mRNA] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. miRNA microarray and mRNA array experiments were performed, qRT-PCR validation for miRNAs and mRNAs. We performed a joint analysis of differentially expressed miRNAs and mRNAs, and proved that the target gene of miRNA-146b is Tbc1d14. It further confirmed that miR-146b targeting tbc1d14 regulates brucella-mediated autophagy of RAW264.7 cells. Finally, the molecular mechanism of tbc1d14 influencing brucella-mediated autophagy of RAW264.7 cells was preliminarily revealed by DGE sequencing.
|
|
|
Overall design |
In this study,We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. Three samples from each of the two groups were selected for the mRNA microarray.
|
|
|
Contributor(s) |
Jiao H |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Feb 10, 2019 |
Last update date |
Feb 13, 2019 |
Contact name |
Xie qin |
E-mail(s) |
qxie@lc-bio.com
|
Phone |
15618926050
|
Organization name |
LC sciences
|
Street address |
Minchi first road, pujiang town, minhang district, Shanghai
|
City |
shanghai |
ZIP/Postal code |
200000 |
Country |
China |
|
|
Platforms (1) |
GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
|
Samples (6)
|
GSM3596912 |
per1 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
GSM3596913 |
per2 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
GSM3596914 |
per3 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h |
GSM3596915 |
M5_90_1 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
GSM3596916 |
M5_90_2 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
GSM3596917 |
M5_90_3 from RAW264.7 cells infected with B. melitensis M5-90 for 4h |
|
This SubSeries is part of SuperSeries: |
GSE126499 |
The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 |
|
Relations |
BioProject |
PRJNA521652 |