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Series GSE126343 Query DataSets for GSE126343
Status Public on Feb 11, 2019
Title The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 [mRNA]
Organism Mus musculus
Experiment type Expression profiling by array
Summary We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. miRNA microarray and mRNA array experiments were performed, qRT-PCR validation for miRNAs and mRNAs. We performed a joint analysis of differentially expressed miRNAs and mRNAs, and proved that the target gene of miRNA-146b is Tbc1d14. It further confirmed that miR-146b targeting tbc1d14 regulates brucella-mediated autophagy of RAW264.7 cells. Finally, the molecular mechanism of tbc1d14 influencing brucella-mediated autophagy of RAW264.7 cells was preliminarily revealed by DGE sequencing.
 
Overall design In this study,We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. Three samples from each of the two groups were selected for the mRNA microarray.
 
Contributor(s) Jiao H
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 10, 2019
Last update date Feb 13, 2019
Contact name Xie qin
E-mail(s) qxie@lc-bio.com
Phone 15618926050
Organization name LC sciences
Street address Minchi first road, pujiang town, minhang district, Shanghai
City shanghai
ZIP/Postal code 200000
Country China
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM3596912 per1 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h
GSM3596913 per2 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h
GSM3596914 per3 from RAW264.7 cells infected with B. melitensis M5-90 ∆per for 4h
This SubSeries is part of SuperSeries:
GSE126499 The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90
Relations
BioProject PRJNA521652

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126343_RAW.tar 23.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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