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Series GSE12629 Query DataSets for GSE12629
Status Public on Sep 01, 2008
Title Analysis of copy number change in cord lining epithelial cells after phage integrase mediated genomic integration.
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary The two obstacles that impede a wider application of genetically modified cells expressing therapeutic transgenes for ex vivo gene therapy are the immune mediated rejection of the transplanted cells, combined with their potential to cause iatrogenic oncogenesis. In this study we describe a new cellular vehicle for this form of therapy, termed the cord lining epithelial cell (CLEC). CLECs are derived from the human amnion and incorporate many of the immunoregulatory functions associated with the fetal/maternal interface. We show that CLECs can be safely transfected by phage φC31 integrase to accomplish site-specific integration of a therapeutic human transgene. We also show that transplanted CLECs are not oncogenic in vivo and can be maintained in immunocompetent mice where acute xeno-rejection rapidly destroys other human cell types. Finally, we demonstrate the utility of CLECs for ex vivo gene therapy by delivering human coagulation factor 8 to mice with Hemophilia A.
Overall design High-resolution copy number profiling was performed on genomic DNA of untreated (GSM315546 and GSM315713) and phage integrase modified CLECs (GSM315974 and GSM316895) using the Human Mapping 500K Array Set (Affymetrix) and the data analyzed using GeneChip Chromosome Copy Number Analysis Tool. Regions of copy number gain or loss were defined as having 3 consecutive SNPs concordant for significant copy number abnormalities. Log2 signal intensity ratios >0.3 and <-0.3 were criteria for significant copy number gain and loss, respectively.
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Submission date Aug 29, 2008
Last update date May 17, 2017
Contact name Jaichandran Sivalingam
Organization name National Cancer Center
Department Division of Medical Sciences
Lab Laboratory of Applied Human Genetics
Street address 11 Hospital Drive
City Singapore
ZIP/Postal code 169610
Country Singapore
Platforms (2)
GPL3718 [Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array
GPL3720 [Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array
Samples (4)
GSM315546 Human Umbilical Cord lining Epithelial cells 1
GSM315713 Human Umbilical Cord lining Epithelial cells 2
GSM315974 GFP integrated Human Umbilical Cord lining Epithelial cells 1
BioProject PRJNA112801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12629_RAW.tar 86.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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