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Series GSE126277 Query DataSets for GSE126277
Status Public on Feb 13, 2019
Title IFN-α-producing proliferating myeloid cells reveal type I IFN-stimulating gene signature
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We previously established a method to generate myeloid lineage cells with proliferation capacity from induced pluripotent stem cells (iPSCs) (Zhang R. Cancer Immunol Res. 3: 668, 2015). In this study, we generated the IFN-α-producing cells by genetic engineering of mouse iPSC-derived myeloid cells (iPSC-pMCs). Gene expression profiling of IFN-α-producing iPSC-pMCs (IFN-α-iPSC-pMCs) revealed a distinct interferon-stimulated gene (ISG) signature: of 613 differentially expressed genes (versus iPSC-pMC), 345 genes were ISGs of which > 70 % were type I IFN-related. Gene ontology analysis showed that the up-regulated gene signature was enriched for transcripts associate with immune responses, cellular responses to type I IFN, and defense responses to virus.
 
Overall design Gene expression profiles of iPSC-pMCs, IFN-α-transuduced iPSC-pMCs, and IFN-α-treated iPSC-pMCs.
 
Contributor(s) Uemura Y, Zhang R
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Submission date Feb 08, 2019
Last update date Mar 21, 2019
Contact name Ranmaru Shimoda
E-mail(s) shimoda@rhelixa.com
Phone +818043368250
Organization name Rhelixa, Inc.
Street address 3F Yayoi Bldg., 3-7-4 Iwamotocho
City Chiyoda
State/province Tokyo
ZIP/Postal code 101-0032
Country Japan
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3595651 pMC_01
GSM3595652 pMC_02
GSM3595653 IFN-alpha-pMC_01
This SubSeries is part of SuperSeries:
GSE126281 Type I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+ Dendritic Cells
Relations
BioProject PRJNA521844
SRA SRP185724

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126277_RAW.tar 3.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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