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Series GSE126151 Query DataSets for GSE126151
Status Public on Nov 08, 2019
Title A systematic analysis of nuclear heat-shock protein 90 identifies a metazoan-specific regulatory module
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Molecular chaperones such as heat-shock proteins (HSPs) help in protein folding and complex assembly processes. Their role in cytosol has been very well elucidated. Chaperones are also present in the nucleus, a compartment where proteins enter after being fully folded in the cytosol, raising an important question about chaperones function in this compartment. We have performed a systematic analysis of nuclear heat- shock protein 90 to identify regulatory functions of this chaperone in the nucleus. Combining physical and genetic interactomes with a cancer co-expression screen allowed us to define 'core functional interactors' of nuclear HSP90 consisting of five proteins. Using transcriptional studies we identified Host Cell Factor C1 (HCFC1) as a metazoan-specific transcriptional regulator that depends on HSP90 for its stability in the nucleus. We found that HSP90 is required for optimal activity of HCFC1 in transcription. Thus our study provides the first global insight into the function of nuclear HSP90.
Overall design ChIP-Seq and RNA-Seq
Contributor(s) Antonova A, Hummel B, Sawarkar R
Citation(s) 31693902
Submission date Feb 06, 2019
Last update date Nov 08, 2019
Contact name Barbara Hummel
Organization name Max Planck Institute of Immunobiology and Epigenetics
Street address Stübeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM3592363 HSP90_ChIP-seq
GSM3592364 HSP90_Input
GSM3592365 K562_Control_Rep1
BioProject PRJNA521153
SRA SRP184211

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE126151_K562_HSP90_ChIPseq_peaks.bed.gz 20.9 Kb (ftp)(http) BED
GSE126151_RawCounts_HPC7_HSP90i.txt.gz 474.8 Kb (ftp)(http) TXT
GSE126151_RawCounts_K562_HSP90i.txt.gz 692.4 Kb (ftp)(http) TXT
GSE126151_RawCounts_MEF_HSP90i.txt.gz 483.9 Kb (ftp)(http) TXT
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