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| Status |
Public on Apr 23, 2019 |
| Title |
Deletion of the KH1 domain coding sequence of Fmr1 leads to transcriptional alterations and attentional deficits in rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that the previously published Fmr1 knockout rat model of FXS expresses an Fmr1 transcript with an in-frame deletion of exon 8, which encodes for the K-homology (KH) RNA-binding domain, KH1. We observed that the deletion of exon 8 in 10 male rats within the medial prefrontal cortex (mPFC) led to transcriptional alterations compared to 12 WT rats using RNAseq. Additionally, we used weighted gene co-expression network analysis to generate 23 modules specific to the mPFC with tissue from 35 WT rat samples.
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| |
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| Overall design |
RNAseq using WT and Fmr1 delta exon 8 mPFC samples
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| |
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| Contributor(s) |
Golden CE, Breen MS, Koro L, Sonar S, Niblo K, Browne A, Burlant N, Di Marino D, De Rubeis S, Baxter MG, Buxbaum JD, Harony-Nicolas H |
| Citation(s) |
30877790 |
| |
| Submission date |
Feb 04, 2019 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Michael S Breen |
| Organization name |
Ichan School of Medicine at Mount Sinai
|
| Department |
Depts. of Psychiatry, Genetics and Genomic Sciences
|
| Street address |
1468 Madison Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10669 |
Illumina Genome Analyzer IIx (Rattus norvegicus) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA520888 |
| SRA |
SRP183479 |
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