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Series GSE125983 Query DataSets for GSE125983
Status Public on Feb 01, 2019
Title Gene expression in the small intestine of mice colonized with Pseudomonas aeruginosa
Organism Mus musculus
Experiment type Other
Summary Celiac disease (CeD) is a food sensitivity characterized by a breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the underlying mechanisms are incompletely understood. To evaluate a functional role of bacterial proteases in CeD, we colonized germ-free or clean SPF mice with P.aeruginosa PA14 or P.aeruginosa PA14 disrupted in lasB gene (elastase). We show gluten-independent, PAR-2 mediated upregulation of inflammatory pathways by LasB in C57BL/6 mice without villus blunting. In mice expressing CeD risk genes, P. aeruginosa elastase synergized with gluten to induce more severe inflammation that was associated with moderate villus blunting.
 
Overall design Determination of inflammatory genes in tissue and intraepithelial lymphocytes of mice. For RNA extraction, RNeasy Mini Kit (Qiagen) was used. Gene Expression CodeSet for mouse inflammation genes (Mouse Inflammation panel, 254 genes) were run according to the manufacturer’s instructions (NanoString Technologies Inc.). The results obtained were analyzed with nSolver 2.5 (NanoString Technologies).
 
Contributor(s) Caminero A, Verdu E
Citation(s) 30867416
Submission date Jan 31, 2019
Last update date May 03, 2019
Contact name Alberto Caminero
E-mail(s) acamine@mcmaster.ca
Organization name McMaster University
Street address 1280 Main St W
City Hamilton
State/province ON
ZIP/Postal code L8S 4L8
Country Canada
 
Platforms (1)
GPL26138 NanoString nCounter Mouse Gene Expression Custom CodeSet
Samples (45)
GSM3587311 Tissue C57 GF mice monocolonized P.aeruginosa LasB 1
GSM3587312 IELs C57 GF mice monocolonized P.aeruginosa PA14 1
GSM3587313 IELs C57 GF mice monocolonized P.aeruginosa PA14 2
Relations
BioProject PRJNA518152

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125983_RAW.tar 190.0 Kb (http)(custom) TAR (of RCC)
Processed data included within Sample table
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