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Series GSE125971 Query DataSets for GSE125971
Status Public on Aug 20, 2019
Title Mapping Histone Modifications in Single Cells Using an Antibody-guided Transposase
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We describe a novel method of mapping genome-wide distributions of epigenetic marks in single cells using a Tn5 transposase complex conjugated to Protein A. This construct is guided to chromatin by an associated antibody, allowing sequence tag insertion and chromatin fragmentation specifically at genomic sites presenting the relevant antigen. This method is capable of processing thousands of individual cells in a single day of bench work.
 
Overall design a novel method of mapping genome-wide distributions of epigenetic marks in single cells using a Tn5 transposase complex conjugated to Protein A
 
Contributor(s) Benjamin C, Ku W, Kang J, Hu G, Perrie J, Tang Q, Zhao K
Citation(s) 31431618
Submission date Jan 31, 2019
Last update date Aug 22, 2019
Contact name Keji Zhao
E-mail(s) zhaok@nhlbi.nih.gov
Organization name national Institute of Health
Department National Heart, Lung, and Blood Institute
Street address Building 10 Room 7B06A
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (96)
GSM3587014 human HEK293T H3K4me3 - 1
GSM3587015 human HEK293T H3K4me3 - 2
GSM3587016 human HEK293T H3K4me3 - 3
Relations
BioProject PRJNA518134
SRA SRP183082

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125971_barcode_96.txt.gz 501 b (ftp)(http) TXT
GSE125971_barcode_name_conversion_table.xlsx 9.1 Kb (ftp)(http) XLSX
GSE125971_peaks_15756_HEK293T.txt.gz 117.5 Kb (ftp)(http) TXT
GSE125971_scH3K4me3_1246_cells_counts_at_293T.txt.gz 1.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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