GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE125740 Query DataSets for GSE125740
Status Public on Jan 29, 2019
Title Fusion of reprogramming factors alters trajectory of somatic lineage conversion
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to the transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce a transient acquisition of pluripotency before establishing the neural fate. We employed genetic lineage tracing and found that induction of iNSCs with individual vectors leads to direct lineage conversion. In contrast, polycistronic expression produces a Brn4-Klf4 fusion protein that enables the induction of pluripotency. Our study demonstrates that a combination of pluripotency and tissue-specific factors allows for direct somatic cell transdifferentiation, bypassing the acquisition of a pluripotent state. This result has major implications for lineage conversion technologies, which hold potential for providing a safer alternative to iPSCs for clinical application both in vitro and in vivo.
Overall design Here we perform RNA-Seq experiments in induced neural stem cells.
Contributor(s) Velychko S, Kang K, Park C, Schöler HR, Han DW
Citation(s) 30943410
Submission date Jan 28, 2019
Last update date Apr 09, 2019
Contact name Chanhyeok Park
Organization name Konkuk University
Department Stem cell and regeneration biotechnology
Lab Hong lab
Street address 120, Neungdong-ro
City Seoul
ZIP/Postal code 05066
Country South Korea
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (7)
GSM3581267 MEFs
GSM3581268 cNSCs
GSM3581269 pOKSM
BioProject PRJNA517430
SRA SRP182659

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125740_all_genes.fpkm_table.txt.gz 490.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap