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Series GSE125623 Query DataSets for GSE125623
Status Public on Aug 27, 2019
Title Analysis of local glucocorticoid receptor occupancy at glucocorticoid-repressed inflammatory enhancers in Beas-2B airway epithelial cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary This study combined ChIP-seq using two different GR antibodies and stable GR knockdown in Beas-2B airway epithelial cells to determine whether glucocorticoid-mediated repression of inflammatory enhancers requires local GR occupancy
Overall design Duplicate samples of Beas-2B cells transduced with lentiviral-shRNA against GR (shGR) or a control construct (shCtrl) were treated with vehicle (ethanol), TNF (20 ng/ml), dexamethasone (100 nM) or TNF+dex for one hour prior to chromatin immunoprecipitation with one of two GR antibodies and sequencing. Duplicate Input DNA samples, representing both shCtrl and shGR groups, were included as controls.
Contributor(s) Sasse SK, Gruca M, Allen MA, Kadiyala V, Song T, Gally F, Gupta A, Pufall MA, Dowell RD, Gerber AN
Citation(s) 31519741
Submission date Jan 24, 2019
Last update date Nov 26, 2019
Contact name Sarah Sasse
Organization name National Jewish Health
Department Medicine
Lab Anthony Gerber
Street address 1400 Jackson St., K720
City Denver
State/province CO
ZIP/Postal code 80206
Country USA
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (36)
GSM3579032 GR-IA1_Ctrl_V1
GSM3579033 GR-IA1_Ctrl_V2
GSM3579034 GR-IA1_Ctrl_T1
BioProject PRJNA516938
SRA SRP181951

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Supplementary file Size Download File type/resource
GSE125623_RAW.tar 11.4 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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