GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE125519 Query DataSets for GSE125519
Status Public on Jan 27, 2020
Title GPCR-induced YAP activation sensitizes fibroblasts to profibrotic effects of TGFβ1
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Fibrosis is an uncontrolled wound healing process resulting from tissue injury, inflammation and fibroblast activation, finally leading to accumulation of extracellular matrix (ECM) components in tissues and to organ failure. The underlying mechanisms of fibrosis have been intensely studied and it has become clear that multiple pathways and their crosstalk regulate fibrotic diseases with a few central players, including TGFβ1. Recently, YAP and TAZ were implicated in fibrosis as one of the essential components of its pathogenesis. YAP/TAZ are known to crosstalk with the TGFβ pathway in the nucleus and regulate its activity on transcriptional level by binding to Smad2/3/4 complexes. We hypothesized that factors, such as GPCR ligands which regulate YAP/TAZ, might modulate the profibrotic responses to TGFβ1. We performed gene expression microarray in NHDF treated with TGFβ1, LPA, the combination of TGFβ1/LPA or vehicle. The aim was to have a broader look at transcriptional signatures induced by the individual treatments and in the combination of TGFβ1 and LPA.
Overall design Comparison of primary human skin fibroblasts stimulated with TGFβ1, LPA and the combination of TGFβ1 plus LPA; 4 replicates per group
Contributor(s) Zmajkovicova K, Bauer Y, Schnoebelen M, Freti D, Boucher M, Renault B, Studer R, Birker M, Klenk A, Nayler O, Gatfield J
Citation(s) 32053631
Submission date Jan 23, 2019
Last update date Mar 02, 2020
Contact name Axel Klenk
Organization name Idorsia Pharmaceuticals Ltd.
Department Research Informatics
Street address Hegenheimermattweg 91
City Allschwil
ZIP/Postal code 4123
Country Switzerland
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (16)
GSM3576309 R3 LPA
GSM3576310 R4 Vehicle
GSM3576311 R1 LPA.TGFB
BioProject PRJNA516663

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125519_RAW.tar 341.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap