NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE12543 Query DataSets for GSE12543
Status Public on Aug 26, 2008
Title Profiles and ChIP on chip experiments with dl1500 virus (expressing WT small e1a), R2G and deltaCR2 mutant viruses
Organism Homo sapiens
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary Adenovirus e1a induces quiescent human cells to divide. We found that e1a causes global relocalization of the RB-proteins (RB, p130, p107) and p300/CBP histone acetyltransferases on promoters that restricts H3K18ac to a limited set of genes to stimulate cell cycling and inhibit antiviral responses and cellular differentiation. Soon after expression, e1a binds transiently to cell cycle/growth genes, causing enrichment of p300/CBP, PCAF, H3K18ac, depletion of RB-proteins, and transcriptional activation. e1a also associates transiently with antiviral genes, causing enrichment for RB, p130, H4K16ac, increased nucleosome density, and repression. At later times, e1a and p107 bind mainly to development/differentiation genes, repressing transcription. The temporal order of e1a binding required its interactions with p300/CBP and RB-proteins. Our data uncover a defined transcriptional and epigenetic reprogramming leading to cellular transformation.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Contributor(s) Ferrari R, Pellegrini M, Horwitz G, Xei W, Berk A, Kurdistani S
Citation(s) 18719284
Submission date Aug 25, 2008
Last update date Dec 28, 2012
Contact name Siavash K Kurdistani
E-mail(s) Skurdistani@mednet.ucla.edu
Organization name UCLA
Department Biological Chemistry
Lab Kurdistani
Street address 615 Charles E Young Dr South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (2)
GPL7031 Agilent Whole Human Genome Oligo Microarray - custom annotation
GPL7032 Human Promoter ChIP-on-chip Microarray - custom annotation
Samples (30)
GSM304305 Expression profile 6 hours post infection for dl1500 virus expressing WT small e1a
GSM304306 Expression profile 12 hours post infection for dl1500 virus expressing WT small e1a
GSM304307 Expression profile 24 hours post infection for dl1500 virus expressing WT small e1a
This SuperSeries is composed of the following SubSeries:
GSE12045 Expression profiles and ChIP on chip genomewide experiments with dl1500 virus (expressing WT small e1a).
GSE12046 Expression profiles and ChIP on chip genomewide experiments with R2G mutant virus
GSE12047 Expression profiles and ChIP on chip genomewide experiments with deltaCR2 mutant virus
Relations
BioProject PRJNA112903

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12543_RAW.tar 2.1 Gb (http)(custom) TAR (of TSV, TXT)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap