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Series GSE125407 Query DataSets for GSE125407
Status Public on Jan 22, 2019
Title Characterization of microRNA-493-5p targets in liver cell lines (2nd set of data)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Primary hepatic tumors mainly include hepatocellular carcinoma (HCC), which is one of the most frequent causes of cancer-related deaths worldwide. Thus far, HCC prognosis has remained extremely poor given the lack of effective treatments. Numerous studies have described the roles played by microRNAs (miRNAs) in cancer progression and the potential of these small noncoding RNAs for diagnostic or therapeutic applications. The current consensus supports the idea that direct repression of a wide range of oncogenes by a single key miRNA may critically affect the malignant properties of cancer cells in a synergistic manner. In this study, we aimed to investigate the oncogenes controlled by miR-493-5p, a major tumor suppressor miRNA that inactivates miR-483-3p oncomir in hepatic cancer cells. Using global gene expression analysis, we highlighted a set of candidate genes potentially regulated by miR-493-5p. In particular, the canonical MYCN proto-oncogene (MYCN) appeared to be an attractive target of miR-493-5p given its significant inhibition via 3’-UTR targeting in miR-493-5p-rescued HCC cells. We showed that MYCN was overexpressed in liver cancer cell lines and clinical samples from HCC patients. Notably, MYCN expression levels were inversely correlated with miR-493-5p in tumor tissues. We confirmed that MYCN knockdown mimicked the anti-cancer effect of miR-493-5p by inhibiting HCC cell growth and invasion, whereas MYCN rescue hindered miR-493-5p activity. In summary, miR-493-5p is a pivotal miRNA that modulates various oncogenes after its re-expression in liver cancer cells, suggesting that tumor suppressor miRNAs with a large spectrum of action may provide valuable tools for miRNA replacement therapies.
 
Overall design To identify miR-493-5p targets, Hep3B and HepG2 cells were transfected using miR-493-5p mimics and negative control miRNA mimics.
Web link https://onlinelibrary.wiley.com/doi/abs/10.1111/cas.14292
 
Contributor(s) Gailhouste L, Ochiya T
Citation(s) 31883160
Submission date Jan 21, 2019
Last update date Jan 01, 2020
Contact name Luc Gailhouste
E-mail(s) luc.gailhouste@riken.jp
Organization name RIKEN
Department Cluster for Pioneering Research
Lab Liver Cancer Prevention Research Unit
Street address 408 Main Research Building, 2-1 Hirosawa
City Wako
State/province Saitama
ZIP/Postal code 351-0198
Country Japan
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (4)
GSM3573454 Hep3B_control (2nd)
GSM3573455 Hep3B_miR-493-5p (2nd)
GSM3573456 HepG2_control (2nd)
Relations
BioProject PRJNA516240

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125407_RAW.tar 48.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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