GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE125389 Query DataSets for GSE125389
Status Public on Jan 22, 2019
Title Anti-apoptotic regulation contributes successful nuclear reprogramming using cryopreserved oocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT)- mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery effects of melatonin on cryo-damage in cloned embryos. Development of SCNT embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation of 8 pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1, P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin, an agent that reduces apoptosis and ROS production, enhanced blastocyst formation rates in the SCNT-CROP group when compared with the melatonin-untreated group. Additionally, melatonin-treatment increased the derivation efficiency of pluripotent stem cells from cloned embryos using cryopreserved oocyte.
Overall design Here we perform RNA-Seq experiment in melatonin-treated mouse 2-cell and blastocyst embryos, respectively.
Contributor(s) Lee DR, Lee AR, Park CH, Hong K
Citation(s) 30799275
Submission date Jan 21, 2019
Last update date Mar 23, 2019
Contact name Chanhyeok Park
Organization name Konkuk University
Department Stem cell and regeneration biotechnology
Lab Hong lab
Street address 120, Neungdong-ro
City Seoul
ZIP/Postal code 05066
Country South Korea
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (7)
GSM3573256 SCNT_FOC+K_1
GSM3573257 SCNT_FOC+K_2
GSM3573258 SCNT_CROC+K_1
BioProject PRJNA516198
SRA SRP181026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125389_all_genes.fpkm_table.txt.gz 502.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap