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| Status |
Public on Jan 19, 2019 |
| Title |
Noninvasive ultrasound stimulation of the spleen to treat inflammatory arthritis - single cell RNA sequencing of splenic leukocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this study was to characterize splenic leukocytes in arthritic and non-arthritic mice with and without ultrasound treatment
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| Overall design |
Method: Splenocytes were sorted for live, singlet CD45+ cells which were made into libraries using the 10X Genomics Chromium Single Cell 3’ Library & Gel Bead Kit (v2) and sequenced on an Illumina NovaSeq Sequencer.
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| Contributor(s) |
Zachs DP, Offutt SJ, Graham RS, Kim Y, Mueller J, Auger JL, Schuldt NJ, Kaiser CW, Heiller AP, Dutta R, Guo H, Alford JK, Binstadt BA, Lim HH |
| Citation(s) |
30862842 |
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| Submission date |
Jan 18, 2019 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Rachel Stegeman Graham |
| E-mail(s) |
rgraham@umn.edu
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| Phone |
7632277202
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| Organization name |
University of Minnesota
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| Department |
Pediatrics
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| Lab |
Binstadt
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| Street address |
2101 6th St SE
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| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55414 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA515851 |
| SRA |
SRP180322 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125316_RAW.tar |
2.5 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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