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Series GSE125316 Query DataSets for GSE125316
Status Public on Jan 19, 2019
Title Noninvasive ultrasound stimulation of the spleen to treat inflammatory arthritis - single cell RNA sequencing of splenic leukocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study was to characterize splenic leukocytes in arthritic and non-arthritic mice with and without ultrasound treatment
Overall design Method: Splenocytes were sorted for live, singlet CD45+ cells which were made into libraries using the 10X Genomics Chromium Single Cell 3’ Library & Gel Bead Kit (v2) and sequenced on an Illumina NovaSeq Sequencer.
Contributor(s) Zachs DP, Offutt SJ, Graham RS, Kim Y, Mueller J, Auger JL, Schuldt NJ, Kaiser CW, Heiller AP, Dutta R, Guo H, Alford JK, Binstadt BA, Lim HH
Citation(s) 30862842
Submission date Jan 18, 2019
Last update date Mar 20, 2019
Contact name Rachel Stegeman Graham
Phone 7632277202
Organization name University of Minnesota
Department Pediatrics
Lab Binstadt
Street address 2101 6th St SE
City Minneapolis
State/province MN
ZIP/Postal code 55414
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM3568820 s01
GSM3568821 s02
GSM3568822 s03
BioProject PRJNA515851
SRA SRP180322

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125316_RAW.tar 2.5 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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