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Series GSE125130 Query DataSets for GSE125130
Status Public on Jan 16, 2019
Title Phytochrome-based extracellular matrix with reversibly tunable mechanical properties
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Interrogation and control of cellular fate and function using optogenetics is providing revolutionary insights into biology. Optogenetic control of cells is achieved by coupling genetically encoded photoreceptors to cellular effectors and enables unprecedented spatiotemporal control of signaling processes. Here, a fast and reversibly switchable photoreceptor is used to tune the mechanical properties of polymer materials in a fully reversible, wavelength-specific, and dose- and space-controlled manner. By integrating engineered cyanobacterial phytochrome 1 into a polyethylene glycol matrix, hydrogel materials responsive to light in the cell-compatible red/far-red spectrum are synthesized. These materials are applied to study in human mesenchymal stem cells how different mechano-signaling pathways respond to changing mechanical environments, and to control the migration of primary immune cells in 3D. This optogenetics-inspired matrix allows addressing fundamental questions of how cells react to dynamic mechanical environments. Further, remote control of such matrices could create new opportunities for tissue engineering or provide a basis for optically stimulated drug depots.
Overall design Analysis of global gene expression changes due to differences in the mechanical properties of the phytochrome-based hydrogels
Contributor(s) Hörner M, Weber W
Citation(s) 30687975
Submission date Jan 15, 2019
Last update date Mar 27, 2019
Contact name Barbara Hummel
Organization name Max Planck Institute of Immunobiology and Epigenetics
Street address Stübeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (78)
GSM3564026 660nm(1-24h)_660nm(25-48h)_Rep1
GSM3564027 660nm(1-24h)_660nm(25-48h)_Rep2
GSM3564028 660nm(1-24h)_660nm(25-48h)_Rep3
BioProject PRJNA515303
SRA SRP179648

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125130_ReadCounts_alternating_illumination.txt.gz 1.2 Mb (ftp)(http) TXT
GSE125130_ReadCounts_control1_non-photo_responisve_hydrogel.txt.gz 527.0 Kb (ftp)(http) TXT
GSE125130_ReadCounts_control2_PEG_Cph1_coated_glass_surface.txt.gz 533.7 Kb (ftp)(http) TXT
GSE125130_ReadCounts_illumination.txt.gz 946.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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