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Series GSE125109 Query DataSets for GSE125109
Status Public on Jan 01, 2020
Title Tri-cultures of HGSOC cells replicate tissue remodelling observed in biopsies.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We built novel tri-cultures of HGSOC cells with human primary omental adipocytes and fibroblasts which replicated tissue remodelling and matrisome production observed in biopsies. RNAseq analysis showed that tri-cultures reproduced the prognostic matrisome signature.
Overall design RNA sequencing on 1) 3D-tricultures of HGSOC malignant cells, omental fibroblasts and adipocytes with (n = 2) or without (n = 2) TGFβRI + GLI1/2 inhibitors, or on adipocyte-only gels (n = 1), or co-cultures of HGSOC malignant cells and omental fibroblasts (n = 2); and 2) on two HGSOC malignant cell lines grown in monolayer (n = 2-3) and as spheroids (n = 2-3).
Contributor(s) Delaine-Smith R, Maniati E, Malacrida B, Balkwill F
Citation(s) 34189438
Submission date Jan 15, 2019
Last update date Jul 08, 2021
Contact name Eleni Maniati
Organization name Queen Mary University of London
Department Barts Cancer Institute
Lab Cancer and Inflammation
Street address John Vane Science Centre, Charterhouse Square
City London
ZIP/Postal code EC1M 6BQ
Country United Kingdom
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (17)
GSM3563554 RI1
GSM3563555 RI2
GSM3563556 RB
BioProject PRJNA515261
SRA SRP179510

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125109_RPKM_cqn_monocultures_v4.txt.gz 945.6 Kb (ftp)(http) TXT
GSE125109_RPKM_cqn_tricultures_v2.txt.gz 860.5 Kb (ftp)(http) TXT
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