GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE125030 Query DataSets for GSE125030
Status Public on Jan 15, 2019
Title miRNA data from melanoma-derived exosomes and melanoma cell lines
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Identify miRNAs enriched or overexpressed in melanoma-derived exosomes compared to melanoma cells. We analyzed the 2,578 human miRNAs located in the array according to two ways. The first one identified the differentially expressed miRNAs between melanoma-derived exosomes and their parent cells. In this way, we found 198 miRNAs up-regulated in melanoma cell lines compared to their exosomes and 206 miRNAs up-regulated in exosomes compared to their donor cells. The second way of analysis identifies the most expressed miRNAs in the melanoma-derived exosomes without assumption about their expression in parent cells. We defined two criteria; the first one is a RMA (Robust Multi-Array Average, a good alternative to gene expression value) above 5, corresponding to the mean expression level of the array and a SD ≤ 0.2 between all samples to discover miRNAs expressed uniformly between melanoma-derived exosomes M113 and M117. We identified 44 miRNAs under these criteria.
Overall design We used microarray to identified miRNA enriched or overexpressed in melanoma-derived exosomes compared to their parent cells.
Contributor(s) Delphine F
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 14, 2019
Last update date Jan 16, 2019
Contact name Delphine Fradin
Organization name CRCI2NA, Inserm UMR1307, CNRS UMR6075
Street address 8 quai Moncousu
City Nantes
ZIP/Postal code 44007
Country France
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (10)
GSM3561420 melanoma cell line M117, biological rep2
GSM3561421 melanoma-derived exosome M113, biological rep1
GSM3561422 melanoma cell line M113, biological rep1
BioProject PRJNA514998

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE125030_RAW.tar 7.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap