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Series GSE124987 Query DataSets for GSE124987
Status Public on Jan 10, 2020
Title Bone marrow microenvironment mediated drug resistance in Acute promyelocytic leukemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary About 5-10% newly diagnosed and about 20-30% of relapsed acute promyelocytic leukemia (APL) patients will have disease recurrence after receiving currently accepted standards of care. While there are reports of micro-environment mediated drug resistance (EM-DR) in AML, there is no data on the effect of malignant promyelocyte and stromal interaction on Arsenic trioxide (ATO) induced apoptosis. There are limited study available on the effect of leukemic cell interaction on stromal cells. We undertook a preliminary study to evaluate the changes induced by leukemic cells on stromal cells. In a gene expression profiling comparing HS-5 cells in co-culture with NB4 cells alone, 8456 genes were differentially regulated. On a preliminary analysis, we observed an up-regulation of various pathways such as adhesion, Cytokines, Wnt signalling in the stromal cells.
Overall design The HS-5 cells were co-cultured with NB4 cells for 48 hours and the stromal cells were taken out after washing with PBS, characterized by flow cytometry to obstain a purity of more than 95% and subjected to RNA isolation. Similarly HS-5 cells alone were alone used as controls
Contributor(s) Mathews V, Ganesan S, Kumar Palani H
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Submission date Jan 11, 2019
Last update date Jan 12, 2020
Contact name Genotypic technology
Organization name Genotypic Technology
Street address 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
City Bangalore
State/province Karnataka
ZIP/Postal code 560094
Country India
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (6)
GSM3560512 HS-5 alone 1
GSM3560513 HS-5 alone 2
GSM3560514 HS-5 alone 3
BioProject PRJNA514741

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124987_RAW.tar 73.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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