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Series GSE124911 Query DataSets for GSE124911
Status Public on Jun 06, 2019
Title EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits (ATAC-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Memory CD8 T cells have a unique ability to provide lifelong immunity against pathogens. Although memory features generally arise after a challenge with foreign antigen, naïve CD8 single positive (SP) thymocytes may already acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these “innate memory” CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming was secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, we identified direct interaction between EOMES and BRG1 and showed that in vivo acquisition of EOMES-dependent program by CD8SP thymocytes was dependent on this chromatin remodeling factor. In conclusion, our results support a strong epigenetic basis for EOMES-driven establishment of CD8 T cell unconventional memory program.
Overall design CD8SP thymocytes: Innate Memory, Naïve, WT, and Eomes-TG
Contributor(s) Istaces N, Splittgerber M, Silva VL, Nguyen M, Thomas S, Le A, Achouri Y, Calonne E, Defrance M, Fuks F, Goriely S, Azouz A
Citation(s) 31341159
Submission date Jan 10, 2019
Last update date Sep 23, 2019
Contact name Stanislas Goriely
Phone +3226509586
Organization name Université Libre de Bruxelles
Street address Rue Adrienne Bolland 8
City Gosselies
ZIP/Postal code B-6041
Country Belgium
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM3559312 ATAC_Naive
This SubSeries is part of SuperSeries:
GSE124914 EOMES-dependent epigenetic reprogramming during CD8 T cell development leads to acquisition of memory traits
BioProject PRJNA514300
SRA SRP178224

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Supplementary file Size Download File type/resource
GSE124911_RAW.tar 503.5 Mb (http)(custom) TAR (of BIGWIG, NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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